Discussion

Analyses of disease models requires multiplexed and sophisticated imaging technology workflows capable to follow functional molecular dynamics in living systems from molecule, to cell, to tissue, and ultimately in vivo. Such facilities also require safe bio-containment (e.g. BSL2/BSL3) infrastructure. In our efforts at the Institut Pasteur Paris to develop technology workflows for such analyses we will discuss some of the major challenges facing us today. Notably those arising from the increasing need to establish experimental paradigms bearing greater relevance to the biology they aim to study, and especially epidemiological variation.