Discussion

Using phosphospecific flow cytometry we developed a suite of high content, high throughput phenotypic screens on human primary cells. Drug candidates were tested for their modulatory effect on downstream signalling molecules of the stimulated B-cell receptor (BCR) with the aim to identify novel treatment’s. From a small library of molecules we have generated about 250,000 data points across the assays allowing us to create complex phospho profiles that can be interrogated in multiple dimensions with relative ease. This technology has enabled the discovery of several profiles with potential to be of disease relevance and will be further characterised in other assays and systems.