Discussion

3D cultures represent significant challenges when it comes to cell viability assessment following compound or drug treatment. Factors which need to be addressed include whether the reagent used can effectively lyse the 3D microtissue, allowing the necessary components to penetrate to the core of the 3D structure and generate a robust signal which is not quenched by the mass of cells present. This snapshot presentation will focus on recent developments in respect of a 3D culture-optimised viability reagent based around quantitation of the well established live-cell metabolic biomarker ATP.