Discussion

We describe an impedance-based real-time label-free method that can automatically capture the kinetics of the cell mediated or antibody-dependent cell mediated cytolysis( ADCC) of the target cancer cells. Our results show that the impedance –based technology is a label free alternative to the traditional end-point assays. Automated readouts provide direct, sensitive and specific measurements of target cell changes both short term (hrs) and long term (days to weeks). It allows easy quantification of the cell-mediated cytotoxicity and evaluation of the potencies for specific antibodies.