Discussion

Cells grown in defined animal component-free media are susceptible to impaired growth and viability when subjected to stress. Common challenges include plating at low density (Limiting Dilution Cloning and post-cloning outgrowth), recovery from cryopreservation, and growth in semi-solid media. Building on extensive expertise growing cells under defined conditions, SAL Scientific have developed the InstiGRO range of animal component-free media supplements that dramatically improve the growth and viability of cells subjected to routine stress. These supplements have been optimised for use with CHO, HEK and Per.C6 cells lines.