Discussion

The logistics of high throughput screening could be greatly simplified by using cryopreserved cells in multiwell plates. However, conventional cryopreservation techniques when applied to multiwell plates result in low and variable cell viability after thawing. This limits high throughput screening to cells in a chilled liquid format which complicates the planning, delivering and testing. An alternative is to seed multiwell plates from a single frozen source, but this incurs further time and expense.

Asymptote have developed freezing technology which eliminates the variation in cooling rate and ice nucleation in 96 well plates. This ensures high and consistent cell viability across all wells upon thawing. Cryopreserved multiwell plates would effectively decouple manufacture of the plates from the assay process.

Speak with Stephen Lamb or John Morris to find out more and request a sample of IceStart.