Discussion

Background: Biomimesys®, a hyaluronan scaffold have been developed to overcome the 2D flat culture limitations recreating in vivo physiology within the in vitro environment. It allows cell-matrix interaction and cell-cell interaction and recreate a more physiologically authentic 3D architecture of tissues and organs for advanced drug discovery, ADME-Tox profiling, disease modelling

Material and methods: Biomimesys® consists in HA chains with crosslinking agent ADH (adipic dihydrazide acid). We studied the reorganization of cellular cytoskeleton in 3D cell culture and organization of multicellular spheroids within the hydrogel.

Results: Biomimesys® scaffold technology is physiological, easy to use and compatible with all standard analysis methods (OD, microscopy, Immunofluorescence, Western Blot, flow cytometry etc…). Data provide characterization of the hyaluronan scaffold (Young’s modulus, swelling ratio, and porosity), spheroids formation and highlight cell morphology and proliferation rate. Differences and changes in protein expression, gene expression, cytoskeleton architecture (IF) and proliferation rate is compared with a flat 2D culture.

Conclusions: Biomimesys® has also been used successfully with various cell types including cancer cell lines, human primary cells and stem cells. These results highlight the use of Biomimesys® to grow 3D cell culture as a relevant model for metabolism studies and molecules screening.

Key-words: scaffold, 3D cell culture, biomimetic.