Discussion

Multi-parametric cytometrical analysis, such as the determination of cell number or confluency, are important quality control parameters in cell-based assays. To verify optimal cell handling, confirm cell layer integrity and/or determine the health of cells, image-based analysis should be included in cell-based assay workflows. Cell counting offers the added benefit of being able to normalize data to the number of cells in the well being measured, leading to less data variability and improved data integrity.
Here, we show how cytometric parameters can be determined using the EnSight™ Multimode Plate Reader’s brightfield, digital phase and fluorescent imaging modes.