Discussion

A protocol of light stimulation of ChR2 was tested in the Hamamatsu FDSSµCell. For this study, we recorded the calcium ion influx through Cav1.3 with the use of a calcium-sensitive fluorescent dye (Fluo8). A well-known Cav1.3 blocker, Isradipine, was then used to confirm the pharmacological profile for validation. The data obtained for the ChR2/hCav1.3 cell line by light stimulation was also compared to the extracellular potassium stimulus and to patch clamp to cross-check their reliability.