Discussion

Here we present a high density screening of cytochrome P450 on the Fluent™ laboratory automation solution. 384-well microplate format enabled maximum information to be gained while minimizing reagent and compound consumption. Throughput was further increased by simultaneous screening of a number of different CYP enzymes or the use of a combined dose-response/single concentration approach. The close correlation of the experimentally calculated and benchmark IC50 values for known enzyme inhibitors shows the excellent quality of the data generated.