Discussion

MicroRNA engineering has already proved successful in enhancing various CHO cell phenotypes relevant to production of recombinant products. miR-23 has previously been demonstrated to play a role in glutamate metabolism providing an alternative source of critical metabolites for the TCA cycle, ultimately strengthening oxidative metabolism. We will consider how re-programming cellular bioenergetics through miR-23 may allow mammalian production cells to be more productive by favouring metabolic channelling into oxidative metabolism.