Discussion

Trypanosomes are exquisitely ordered and structured cells in which protein localisation can be extremely informative in functional studies of both conserved eukaryotic biology and specific parasite biology. Therefore, we have initiated a project based on high throughput endogenous gene tagging methodologies with the aim of systematically localising all proteins in the trypanosome genome via both N- and C-terminal tagging. Here, we will present data on the first 2000 proteins. Control proteins within our dataset are consistent with previously published localisations and there is also good agreement between N and C-terminal localisation data. We have performed a meta-analysis of this first dataset to inform future strategies. When complete this will be the first flagellated organism to have all its proteins localised.  We are making this data publicly available immediately on a dedicated website (www.tryptag.org) and a summary for each tagged gene will be available on TriTrypDB.

*co-Principal Investigators on TrypTag project along with Keith Gull, Mark Carrington, Sue Vaughan and Christiane Hertz-Fowler. The TrypTag project is funded by the Wellcome Trust as a Wellcome Trust biomedical resource grant 108445/Z/15/Z.