Authors
C Wedel2; K U Förstner1; R Derr2; T N Siegel2;
1 Core Unit Systems Medicine, University of Wuerzburg, Germany; 2 Research Center for Infectious Diseases, University of Wuerzburg, Germany
Discussion
Genome-wide transcription
studies are revealing ever more �dispersed promoters� that unlike �focused
promoters�, lack well-conserved sequence motifs and tight regulation. Dispersed
promoters are nevertheless marked by well-defined chromatin structures, suggesting
that specific sequence elements must exist in these unregulated promoters. Here, we have analyzed regions of transcription initiation in the eukaryotic
parasite Trypanosoma
brucei, in
which RNA polymerase II transcription initiation lacks regulation and occurs
over broad (~10kb) regions without distinct promoter motifs. Using a combination
of site-specific and genome-wide assays, we identified GT-rich promoters that can drive
unidirectional transcription and promote the targeted deposition of the histone
variant H2A.Z. In addition, mapping nucleosome occupancy at high resolution, we
find that nucleosome positioning may affect RNA maturation, pointing to a
previously unrecognized layer of gene regulation in trypanosomes. Our findings show
that even highly dispersed, unregulated promoters contain specific DNA elements
able to induce transcription and changes in chromatin structure.