Abstract

The lack of oxygen supply is associated with a number of
life-threatening diseases such as stroke, myocardial infarction or renal
failure whereby cells are temporarily deprived of O2 and nutrient (ischemia).
Significant cell damage can also occur during the reperfusion phase through
oxidative stress and inflammatory responses. Investigating these pathologies in
vitro requires an experimental set-up capable of rapid deoxygenation, rapid
reperfusion, and parallel monitoring of critical biological parameters
including cellular oxygenation and ROS. The ischemia-reperfusion model
presented here uses a microplate reader with software-controlled programmable
O2 and CO2 regulation in combination with MitoXpress®-Intra, (Luxcel
Biosciences) which enables real-time monitoring of cellular oxygenation. Data
are presented using HepG2 cells and iPS derived cardiomyocytes (Cor.4U®,
Ncardia).