Abstract

Induced pluripotent stem cell (iPSC) technology can be used
to produce cortical neurons and facilitates physiologically relevant disease
modelling and drug discovery. Human disease modelling of the CNS is otherwise
limited due to the lack of availability of human brain tissue and the species differences
between human and animal models.

CENSO Biotechnologies has developed a robust methodology to
produce large scale mature cortical neurons via neuronal rosettes expressing
FOXG1, SOX2 and NESTIN. Firstly we demonstrate in depth characterisation of our
iPSC derived cortical neurons. Secondly we go on to show how iPSC derived
cortical neurons differentiated using CENSO’s protocol can be applied to a high
content screening.

Application of our assay can be used to facilitate
biological research into developmental and neurodegenerative CNS diseases. In
this poster we demonstrate a four day method to measure neuronal differentiation
in 96 and 384 well plates using qualified iPSC-derived cortical neuronal
progenitors