Abstract

To address the need for rapid and sensitive quantitation of protein biomarkers from biological samples, we have developed the FirePlex® Technology Platform. Utilizing patented FirePlex hydrogel particles and a three-region encoding design, FirePlex Immunoaassays allow for true, in-well multiplexing, providing flexible and customizable quantification of analytes. Our high-throughput FirePlex (FirePlex®-HT) Immunoassays quantify up to 10 protein analytes per sample from low sample inputs, in 384-well plate format. The two-step workflow and no-wash assay format limit hands-on time and are amenable to automation. Assay readout is conducted on high-content imagers with data analysis using the FirePlex® Analysis Workbench software, bypassing the need for dedicated instrumentation and expensive software licenses.