Abstract

Human induced pluripotent stem cell (HiPSC) technology combined with robust differentiation methods has the potential to become an integral part of disease research and drug discovery. The ability to differentiate HiPSCs into various cell types provides researchers with a tool to develop tissue specific models of disease and an alternative platform for drug discovery.
Censo Biotechnologies Ltd has optimised a highly reproducible method to differentiate HiPSCs to mature sensory neurons. In order to develop a robust protocol it is important to ensure that the efficacy of each batch of sensory neuron progenitors is of sufficient quality to be used in downstream assays.
Here, we describe a multi-assay procedure to qualify HiPSC derived sensory neurons banks using flow cytometry, high content imaging and qPCR. Neuronal markers specific for early sensory progenitors and mature neurons such as TUJ1, SOX10 and BRN3A were used to monitor differentiation and maturation of the cells.