Abstract

Single
cell cloning (SCC) and associated cloning efficiency (colony outgrowth) is currently regarded as an important and discrete step in stable
cell line development and cell engineering. The advent of new gene
editing technologies, new cell lines, supplements and automated
technologies for single cell cloning are rapidly increasing the effciency of cell
line development. We have previously demonstrated the capabilities and advantages of
the VIPS platform over manual limiting dilution for several cell lines. The
VIPS results in higher seeding efficiencies over manual limiting dilution whilst
giving confidence in clonality thereby reducing the number of plates required for
screening.

In
this poster we use Horizon Discovery’s HD BIOP3 GS Knockout CHO KI cell line in combination with the VIPS seeding and optimised outgrowth
conditions using SAL Scientifc InstiGRO CHO reagents to produce a
significantly higher number of
colonies per plate, thereby reducing the number of plates needing
to
be screened. The experiment was designed to assess the outgrowth of
the
HD-BIOP3 cell line by manual limiting dilution and VIPS under a variety of
conditions.