Abstract
By combining best-in-class resolution with the ability to analyze even
the toughest samples, the Creoptix® WAVE system offers a new level of
performance and flexibility in drug discovery.
Engineered around Grating-Coupled Interferometry (GCI)
1 and
equipped with no-clog microfluidics, the WAVE system offers superior kinetic data
quality across a broader range of samples than traditional SPR. The system can
accurately characterize weak binder kinetics with off-rates of 10/sec, as well
as high affinity antibodies. Additionally, the WAVE’s microfluidics sustain
crude reaction mixtures, viscous detergents and less common solvents, as well
as pure serum, cell supernatant, larger particles and even crude membrane and
VLP extracts.
Here, we show selected examples of GCI applications in the field of low
affinity small molecules and hit screening, including binding kinetics of small
compounds onto self-assembled amyloid fibrils and large proteins (analyte:ligand
MW ratio > 300), and off-rate screening of crude reaction mixtures.
1.-
P. Kozma et. al., “Grating
coupled optical waveguide interferometer for label-free biosensing”, Sensors and
Actuators B: Chemical, 155:446–450 (2011)
