Abstract

Light-sheet fluorescence microscopy (LSFM) provides low out-of-plane photobleaching and phototoxicity, but usually requires two microscope objective lenses orientated at 90° to one another – one for fluorescence excitation and one for fluorescence detection – making it harder to image samples prepared using conventional mounting methods. Oblique plane microscopy (OPM) is a type of LSFM that has been developed in my laboratory and uses a single high numerical aperture microscope objective to provide both fluorescence excitation and detection whilst maintaining the advantages of LSFM, enabling it to provide high-speed 3D imaging for a range of applications on a conventional fluorescence microscope frame.

The speed of OPM imaging can be applied to image a single sample at video volumetric imaging rates. It can also be used to enable higher throughput and time-lapse 3D imaging of arrays of samples arrayed in multi-well plates. This talk will present examples of the application of OPM for high-speed 3D imaging of isolated cardiomyocytes and also examples where the system is being applied to study arrays of multicellular spheroids and organoids in 3D over multiple conditions and over time.