Abstract

Phage display in conjunction with biopanning is a frequently used strategy in the selection process for nanobodies or other expressed binding proteins with specificity to a target antigen. Here we present a fibre optics, SPR based approach that combines both fast kinetic characterization and an efficient selection cycle in a single step. As the assay is based on real-time kinetics monitoring rather than end point characterization such as ELISA, it presents potential for selection and characterization on affinity over avidity.