Abstract

Rapid and flexible plasmid construct generation at scale is one of the most limiting first steps in drug discovery projects. These hurdles can partly be overcome by adopting modular DNA design principles, automated sequence fragmentation and plasmid assembly. To this end we have designed a robust, multi- module golden gate based cloning platform for construct generation with a wide range of applications. To minimize timelines and cost for complex constructs, we developed a software tool named FRAGLER (FRAGment recycLER) that performs codon optimization, multiple sequence alignment and automated generation of fragments for recycling. To highlight the flexibility and robustness of the platform, we fragmented multiple SarsCoV2 spike protein sequences as well as generated protein reagents in a rapid, scalable and cost efficient manner.