Profile of Dr Charlotte Stadler
Charlotte Stadler is a researcher at the Department of Protein Science, Division of Cellular and Clinical Proteomics, KTH Royal Institute of Technology. She is Head of the Cell Profiling facility, located at the Science for Life Laboratory and a researcher within of the Human Protein Atlas (HPA) project. Her research, as well as the expertise of the facility, is focused on antibody-based imaging, as a way to assess spatial information of the human proteome at the cellular and subcellular level. She received her Ph.D. after establishing the systematic pipelines that are the basis of all this work, with Prof. Emma Lundberg and Prof. Mathias Uhlén as her supervisors. She is also devoted to antibody validation and has developed many of the validation schemes used in the HPA for obtaining reliable data on subcellular protein profiling, including a high throughput platform for siRNA mediated knock-down and workflows for recombinant expression validation using GFP-tagging. Her current work focuses on technology development of highly multiplex imaging of tissues and cell samples, and improved protocols for imaging and subcellular profiling of suspension cells such as peripheral blood mononucleated cells (PBMC).