Abstract

Enrichment of leukocytes from blood products like buffy coat, leukocyte reduction system chamber (LRSC), or leukapheresis material such as Leukopak® is time consuming and can potentially influence the physiology of the target cells. Our portfolio of cell separation reagents, the StraightFrom® MicroBeads, were specifically developed to minimize time and
handling steps required for cell isolation from these blood products. This is achieved by eliminating the need to perform density gradient centrifugation or erythrocyte lysis prior to
isolation, which also preserves cell integrity. Based on positive selection, CD3+ T cells, CD4+ T helper cells, CD8+ cytotoxic T cells, CD4+/CD8+ T cells, CD14+ monocytes, CD19+ B cells, or CD56+ NK cells can be isolated from complete buffy coats, LRSCs, or Leukopak® samples within less than 25 minutes with purities above 95%. The positive selection process enables consecutive enrichment of multiple cell populations from a single sample and can be performed in an automated fashion using the MultiMACS™ X. Using various assays, we show that cells were not activated during separation and functionality was preserved. Ultimately, this optimized magnetic cell separation technology allows the isolation of physiologically relevant cells in a quick and easy manner.